8T9S
Structure of gp82 from mycobacteriophage Phaedrus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 83.893, 83.893, 83.893 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.830 - 1.210 |
| R-factor | 0.1677 |
| Rwork | 0.167 |
| R-free | 0.18560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.957 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.320 | 1.270 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.048 | 1.018 |
| Rmeas | 0.050 | 1.046 |
| Rpim | 0.011 | 0.237 |
| Total number of observations | 585771 | 85650 |
| Number of reflections | 30312 | 4417 |
| <I/σ(I)> | 32.1 | 3 |
| Completeness [%] | 99.7 | |
| Redundancy | 19.3 | 19.4 |
| CC(1/2) | 0.999 | 0.855 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | PEG 550 Monomethylether, magnesium chloride, HEPES pH7.5 |
