7SUA
Crystal Structure of the Hypothetical Protein (ACX60_00475) from Acinetobacter baumannii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-E |
| Synchrotron site | APS |
| Beamline | 21-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-03-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.755, 59.784, 75.625 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.060 - 1.650 |
| R-factor | 0.1775 |
| Rwork | 0.176 |
| R-free | 0.20790 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.365 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.069 | 0.687 |
| Rmeas | 0.079 | 0.787 |
| Rpim | 0.037 | 0.379 |
| Number of reflections | 27958 | 1354 |
| <I/σ(I)> | 24.3 | 2.4 |
| Completeness [%] | 99.5 | 99 |
| Redundancy | 4.3 | 4.1 |
| CC(1/2) | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | Protein: 8.8 mg/ml, 0.01M Tris HCl (pH 8.3); Screen: Classics II (D10), 0.1M Bis-Tris (pH 6.5), 20% (w/v) PEG5000 MME |
