7NYJ

Structure of OBP1 from Varroa destructor, form P3<2>21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	P 32 2 1
Unit cell lengths	53.800, 53.800, 91.670
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.590 - 1.810
R-factor	0.18832
Rwork	0.186
R-free	0.22609
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.829
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.600	1.950
High resolution limit [Å]	1.810	1.810
Rmerge	0.080	1.070
Number of reflections	13957	2889
<I/σ(I)>	13.26	1.87
Completeness [%]	100.0	100
Redundancy	20	20
CC(1/2)	0.999	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	2/3 of the initial condition (0.2M Cadmium Chloride, 2.2 ammonium sulfate) + 1/3 of PEG II screen (0.2M calcium chloride, HEPES 0.1M pH 7,5; PEG 4000 30% w/v).