7LYV

Crystal structure of the HCMV pentamer-specific antibody 1-103

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	80
Detector technology	CCD
Collection date	2016-11-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 1 2 1
Unit cell lengths	95.389, 105.240, 102.730
Unit cell angles	90.00, 91.37, 90.00

Refinement procedure

Resolution	46.830 - 1.900
Rwork	0.191
R-free	0.21730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xzc
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.620	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	143460	14384
<I/σ(I)>	5.9	1.8
Completeness [%]	89.9
Redundancy	2
CC(1/2)	0.996	0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	2.1 M sodium formate, 25% PEG 3350, 0.1 M sodium acetate pH 4.5, 0.1 M calcium chloride