6S0O
Crystal Structure of Two-Domain Laccase from Streptomyces griseoflavus produced at 0.25 mM copper sulfate in growth medium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.530, 94.620, 116.500 |
| Unit cell angles | 90.00, 90.39, 90.00 |
Refinement procedure
| Resolution | 45.972 - 1.800 |
| R-factor | 0.1425 |
| Rwork | 0.142 |
| R-free | 0.17940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lhl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.823 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.880 | |
| Number of reflections | 151965 | 11814 |
| <I/σ(I)> | 9.9 | 1.55 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.78 | |
| CC(1/2) | 0.670 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.3M NACL, 0.01 M TRIS-HCL 27,5 % (W/V) PEG 4K, PH 8 |
