6R9Q
Structure of Saccharomyces cerevisiae apo Pan2 pseudoubiquitin hydrolase-RNA exonuclease (UCH-Exo) module in complex with AACCAA RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9159 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 92.189, 117.324, 256.658 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.488 - 3.079 |
| R-factor | 0.2625 |
| Rwork | 0.261 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4czw |
| Data reduction software | XDS |
| Data scaling software | xia2 (1.12) |
| Phasing software | PHASER (2.8) |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.490 | 3.160 |
| High resolution limit [Å] | 3.079 | 3.079 |
| Rmerge | 0.086 | 1.733 |
| Number of reflections | 26217 | 11967 |
| <I/σ(I)> | 12.7 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.6 | 6.3 |
| CC(1/2) | 0.917 | 0.638 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.4 M ammonium phosphate |
