6R9P

Structure of Saccharomyces cerevisiae apo Pan2 pseudoubiquitin hydrolase-RNA exonuclease (UCH-Exo) module in complex with AAUUAA RNA

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9159
Spacegroup name	I 2 2 2
格子定数 [Å]	91.885, 117.091, 255.888
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	86.478 - 2.980
R因子	0.2635
Rwork	0.261
R-free	0.30860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4czw
Data reduction software	XDS
Data scaling software	xia2 (1.12)
Phasing software	PHASER (2.8)
Refinement software	PHENIX (1.14_3260)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	106.450	3.060
分解能 [Å] (高)	2.980	2.980
Rmerge_l_obs	0.065	1.512
独立反射数	28662	4551
<I/σ(I)>	13.5	1.1
完全性 [%]	100.0	100
冗長性	6.6	6.21
CC(1/2)	0.999	0.726

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP		298	0.4 M ammonium phosphate