6R9M
Structure of Saccharomyces cerevisiae apo Pan2 pseudoubiquitin hydrolase-RNA exonuclease (UCH-Exo) module in complex with AAGGAA RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-23 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9159 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 91.414, 118.105, 257.281 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.021 - 3.329 |
R-factor | 0.2399 |
Rwork | 0.237 |
R-free | 0.29530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4czw |
Data reduction software | XDS |
Data scaling software | xia2 (1.12) |
Phasing software | PHASER (2.8) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.170 | 3.420 |
High resolution limit [Å] | 3.329 | 3.329 |
Rmerge | 0.097 | 1.527 |
Number of reflections | 20875 | 1509 |
<I/σ(I)> | 7.3 | 1.2 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 6.5 | 6 |
CC(1/2) | 0.983 | 0.649 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.4 M ammonium phosphate |