6R9J
Structure of Saccharomyces cerevisiae apo Pan2 pseudoubiquitin hydrolase-RNA exonuclease (UCH-Exo) module in complex with A7 RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 91.480, 116.409, 257.833 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.928 - 3.326 |
| R-factor | 0.25 |
| Rwork | 0.247 |
| R-free | 0.29820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4czw |
| Data reduction software | XDS |
| Data scaling software | xia2 (1.12) |
| Phasing software | PHASER (2.8) |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.928 | 3.420 |
| High resolution limit [Å] | 3.326 | 3.326 |
| Rmerge | 0.095 | 1.821 |
| Number of reflections | 20507 | 1481 |
| <I/σ(I)> | 8.3 | 1 |
| Completeness [%] | 99.5 | 98.6 |
| Redundancy | 4.6 | 4.4 |
| CC(1/2) | 0.990 | 0.590 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.4 M ammonium phosphate |
