6QU1
Crystal structure of the KAP1 RBCC domain in complex with the SMARCAD1 CUE1 domain at 3.7 angstrom resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-05 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.92 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 64.496, 64.496, 287.986 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.431 - 3.700 |
R-factor | 0.3036 |
Rwork | 0.300 |
R-free | 0.34640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6h3a |
RMSD bond length | 0.003 |
RMSD bond angle | 0.678 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.431 | 4.140 |
High resolution limit [Å] | 3.700 | 3.700 |
Rmerge | 0.235 | 2.081 |
Rpim | 0.106 | 0.805 |
Number of reflections | 7157 | 1948 |
<I/σ(I)> | 7.3 | 1.4 |
Completeness [%] | 99.7 | 99.4 |
Redundancy | 9.2 | 7.3 |
CC(1/2) | 0.995 | 0.816 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 25 % PEG 3350, 0.1 M HEPES pH 7.5 |