6QM2

NlaIV restriction endonuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-17
Detector	MARRESEARCH
Wavelength(s)	1.05
Spacegroup name	P 62 2 2
Unit cell lengths	110.380, 110.380, 209.006
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.720 - 2.800
R-factor	0.19245
Rwork	0.191
R-free	0.22103
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.083
Data reduction software	XDS
Data scaling software	XDS
Phasing software	autoSHARP
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.970
High resolution limit [Å]	2.800	2.800
Rmerge	0.043	1.052
Number of reflections	18997
<I/σ(I)>	28.92	1.72
Completeness [%]	98.5	98.6
Redundancy	7.5	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	294	10 mg/ml of the enzyme in 15 % glycerol, 50 mM NaOH/HEPES pH 7,5, 50 mM KCl, 10 mM DTT and 5 mM CaCl2 was mixed in 1:1 ratio with double stranded DNA composed of the 5'-ATGGTACCTGC-3' and 5'-CAGGTACCATG-3' strands. The protein-DNA solutions were in turn mixed in 1:1 ratio with the precipitant solution containing 2 M NaCl and 100 mM citric acid, pH 5.