6QF1
X-Ray structure of Proteinase K crystallized on a silicon chip
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2017-09-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| 格子定数 [Å] | 68.467, 68.467, 108.272 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 44.196 - 1.737 |
| R因子 | 0.1351 |
| Rwork | 0.134 |
| R-free | 0.15580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b5l |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.746 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.8) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1-2155_9999) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 44.196 | 1.770 |
| 分解能 [Å] (高) | 1.737 | 1.737 |
| Rmerge_l_obs | 0.143 | 0.646 |
| Rmeas | 0.150 | 0.677 |
| 独立反射数 | 27136 | 1465 |
| <I/σ(I)> | 10.9 | 3.4 |
| 完全性 [%] | 99.6 | 99.9 |
| 冗長性 | 11.1 | 11.3 |
| CC(1/2) | 0.997 | 0.921 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1M CHC buffer pH 6.5, 0.6M ammonium sulphate, 10mM calcium chloride |
