6QD5
X-ray Structure of the Human Urea Channel SLC14A1/UT1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 63 |
Unit cell lengths | 112.331, 112.331, 87.940 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.335 - 2.398 |
R-factor | 0.1777 |
Rwork | 0.175 |
R-free | 0.22510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ezc |
RMSD bond length | 0.009 |
RMSD bond angle | 1.046 |
Data reduction software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.335 | 2.484 |
High resolution limit [Å] | 2.398 | 2.398 |
Rpim | 0.047 | 0.614 |
Number of reflections | 24788 | 2434 |
<I/σ(I)> | 9.02 | 1.29 |
Completeness [%] | 99.8 | 98.66 |
Redundancy | 2 | 2 |
CC(1/2) | 0.996 | 0.475 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 36% PEG400 -- 0.1M tris pH 8.5 -- 0.05M lithium sulfate -- 0.05M sodium sulfate |