6Q76
Complex of rice blast (Magnaporthe oryzae) effector protein AVR-Pia with the HMA domain of Pikp-1 from rice (Oryza sativa)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 34.844, 53.444, 117.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.720 - 1.900 |
R-factor | 0.2053 |
Rwork | 0.203 |
R-free | 0.24510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2myw 5a6p |
RMSD bond length | 0.012 |
RMSD bond angle | 1.501 |
Data reduction software | xia2 (0.5.328) |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHASER (2.7.17) |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.720 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.057 | |
Number of reflections | 18107 | 1151 |
<I/σ(I)> | 19.7 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.6 | 13.3 |
CC(1/2) | 0.990 | 0.810 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.12 M Alcohols (0.2 M 1,6-Hexanediol; 0.2 M 1-Butanol; 0.2 M 1,2-Propanediol; 0.2 M 2-Propanol; 0.2 M 1,4-Butanediol; 0.2 M 1,3-Propanediol), 0.1 M Buffer System 1 (1.0 M imidazole; MES monohydrate (acid), pH 6.5) and 50 % v/v Precipitant Mix 2 (40 % v/v Ethylene glycol; 20 % w/v PEG 8000) |