6Q5O
Crystal structure of a CC-Hex mutant that forms an antiparallel four-helix coiled coil CC-Hex*-LL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-10-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.77999 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 28.868, 29.378, 55.119 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.560 - 1.000 |
| R-factor | 0.17017 |
| Rwork | 0.168 |
| R-free | 0.21193 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.816 |
| Data reduction software | XDS (BUILT20180808) |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.560 | 27.560 | 1.017 |
| High resolution limit [Å] | 1.000 | 2.714 | 1.000 |
| Rmerge | 0.050 | 0.034 | 4.401 |
| Rmeas | 0.054 | 0.037 | 4.769 |
| Rpim | 0.021 | 0.014 | 1.826 |
| Number of reflections | 13041 | 721 | 643 |
| <I/σ(I)> | 15.5 | 61.8 | 0.4 |
| Completeness [%] | 99.9 | 99.6 | 100 |
| Redundancy | 12.5 | 11.4 | 12.8 |
| CC(1/2) | 0.999 | 0.999 | 0.450 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.6 M potassium sodium tartrate tetrahydrate, 50 mM Tris |
