6Q5M
Crystal structure of a CC-Hex mutant that forms an antiparallel four-helix coiled coil CC-Hex*-L24Dab
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-07-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9783 |
| Spacegroup name | P 61 2 2 |
| 格子定数 [Å] | 71.380, 71.380, 35.400 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 35.720 - 1.500 |
| R因子 | 0.19413 |
| Rwork | 0.192 |
| R-free | 0.22386 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.013 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.820 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.7.1) |
| Phasing software | PHASER (7.0.06) |
| Refinement software | REFMAC (5.8.0238) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 35.720 | 35.690 | 1.530 |
| 分解能 [Å] (高) | 1.500 | 8.220 | 1.500 |
| Rmerge_l_obs | 0.048 | 0.015 | 1.173 |
| Rmeas | 0.061 | 0.018 | 1.331 |
| Rpim | 0.036 | 0.009 | 0.619 |
| 独立反射数 | 8775 | 66 | 433 |
| <I/σ(I)> | 12 | 43.1 | 1.1 |
| 完全性 [%] | 98.9 | 89.3 | 100 |
| 冗長性 | 4.4 | 3.9 | 4.4 |
| CC(1/2) | 1.000 | 1.000 | 0.703 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 0.8 M Na/K hydrogen phosphate |
