6Q5J
Crystal structure of a CC-Hex mutant that forms a parallel six-helix coiled coil CC-Hex*-L24E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2018-03-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97628 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 55.073, 55.073, 138.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.190 - 1.690 |
R-factor | 0.21813 |
Rwork | 0.216 |
R-free | 0.25792 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.618 |
Data reduction software | DIALS (1.8.5-g9e40d7de) |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER (2.8.2) |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.320 | 46.320 | 1.730 |
High resolution limit [Å] | 1.690 | 7.560 | 1.690 |
Rmerge | 0.193 | 0.041 | 1.154 |
Rmeas | 0.197 | 0.042 | 1.178 |
Rpim | 0.011 | 0.235 | |
Number of reflections | 24910 | 7200 | 1809 |
<I/σ(I)> | 10.6 | ||
Completeness [%] | 100.0 | 99.7 | 100 |
Redundancy | 24.5 | 19.7 | 24.6 |
CC(1/2) | 1.000 | 1.000 | 0.505 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM NaCl, 50 mM MES buffer and 10% w/v PEG 2000 MME at pH 6.0 |