6Q5I
Crystal structure of a CC-Hex mutant that forms an antiparallel four-helix coiled coil CC-Hex*-L24E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-03-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97628 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 69.070, 69.070, 35.490 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.380 - 1.760 |
| R-factor | 0.1843 |
| Rwork | 0.183 |
| R-free | 0.20731 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.781 |
| Data reduction software | DIALS (1.8.5-g9e40d7de-release) |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 17.380 | 17.380 | 1.810 |
| High resolution limit [Å] | 1.760 | 7.870 | 1.760 |
| Rmerge | 0.205 | 0.051 | 4.738 |
| Rmeas | 0.208 | 0.052 | 4.800 |
| Rpim | 0.034 | 0.010 | 0.763 |
| Number of reflections | 5281 | 75 | 382 |
| <I/σ(I)> | 12.9 | 52.4 | 1 |
| Completeness [%] | 100.0 | 91.2 | 100 |
| Redundancy | 36.5 | 27.3 | 39.2 |
| CC(1/2) | 0.999 | 0.999 | 0.661 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.0 M Ammonium Sulphate and 50 mM Tris |
