6Q5H
Crystal structure of a CC-Hex mutant that forms an antiparallel four-helix coiled coil CC-Hex*-L24D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-03-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 70.200, 70.200, 35.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.870 - 1.200 |
| R-factor | 0.15252 |
| Rwork | 0.151 |
| R-free | 0.17979 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.069 |
| Data reduction software | XDS (Nov 11, 2017) |
| Data scaling software | XDS (Nov 11, 2017) |
| Phasing software | PHASER (7.0.052) |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 15.870 | 15.870 | 1.230 |
| High resolution limit [Å] | 1.200 | 5.370 | 1.200 |
| Rmerge | 0.098 | 0.039 | 4.049 |
| Rmeas | 0.016 | 0.039 | 4.110 |
| Rpim | 0.022 | 0.007 | 0.697 |
| Number of reflections | 16641 | 228 | 1215 |
| <I/σ(I)> | 17.2 | ||
| Completeness [%] | 99.9 | 96.3 | 99.9 |
| Redundancy | 36.3 | 30.3 | 34.3 |
| CC(1/2) | 1.000 | 1.000 | 0.728 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.0 M Ammonium sulfate, 0.05 M Tris. |
