6P0X
Structure of the N-terminal domain of effector protein SpvB from Salmonella typhimurium strain LT2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-15 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 101.351, 101.351, 211.302 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.280 - 2.400 |
R-factor | 0.1887 |
Rwork | 0.187 |
R-free | 0.21480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4igl |
RMSD bond length | 0.003 |
RMSD bond angle | 0.622 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.15.2_3472)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rpim | 0.019 | 0.222 |
Number of reflections | 44119 | 2160 |
<I/σ(I)> | 41.29 | |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 14.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 0.1 M Bis-Tris pH 6.5, 14% poly(acrylic acid) 5100 sodium salt, 4% PEG3350, 1 mM DTT and 20 mM MgCl2 |