6OM8
Caenorhabditis Elegans UDP-Glucose Dehydrogenase in complex with UDP-Xylose
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2017-06-21 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 2 21 21 |
| 格子定数 [Å] | 157.710, 168.170, 279.560 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 52.698 - 2.449 |
| R因子 | 0.1937 |
| Rwork | 0.194 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o3j |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.911 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 52.698 | 2.510 |
| 分解能 [Å] (高) | 2.449 | 2.449 |
| 独立反射数 | 266791 | 19914 |
| <I/σ(I)> | 9.1 | |
| 完全性 [%] | 98.1 | |
| 冗長性 | 6.295 | |
| CC(1/2) | 0.996 | 0.455 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10 mg/mL C. Elegans UDP-Glucose Dehydrogenase Citric acid buffer pH 5.0 200mM Lithium Chloride 4% PEG 8000 |
