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6OM4

The structure of Microcin C7 biosynthetic enzyme MccB in complex with N-formylated MccA

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 21-ID-D
Synchrotron siteAPS
Beamline21-ID-D
Temperature [K]80
Detector technologyCCD
Collection date2018-01-16
DetectorRAYONIX MX-300
Wavelength(s)1.078
Spacegroup nameP 21 21 21
Unit cell lengths55.094, 76.059, 131.902
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution65.980 - 1.700
R-factor0.15692
Rwork0.155
R-free0.19868
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3h9g
RMSD bond length0.013
RMSD bond angle1.700
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHENIX
Refinement softwareREFMAC (5.8.0230)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]65.9801.725
High resolution limit [Å]1.7001.700
Number of reflections598374320
<I/σ(I)>14.2
Completeness [%]96.1
Redundancy14
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP2820.1 M Magnesium formate, 15% w/v Polyethylene glycol 3350, and 6% w/v 1,6-Hexanediol

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건을2024-03-27부터공개중

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