6OM4
The structure of Microcin C7 biosynthetic enzyme MccB in complex with N-formylated MccA
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2018-01-16 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.078 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 55.094, 76.059, 131.902 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 65.980 - 1.700 |
| R因子 | 0.15692 |
| Rwork | 0.155 |
| R-free | 0.19868 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h9g |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.013 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.700 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0230) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 65.980 | 1.725 |
| 分解能 [Å] (高) | 1.700 | 1.700 |
| 独立反射数 | 59837 | 4320 |
| <I/σ(I)> | 14.2 | |
| 完全性 [%] | 96.1 | |
| 冗長性 | 14 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 282 | 0.1 M Magnesium formate, 15% w/v Polyethylene glycol 3350, and 6% w/v 1,6-Hexanediol |
