Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 3 2 |
| 格子定数 [Å] | 228.116, 228.116, 228.116 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 24.889 - 3.850 |
| R因子 | 0.2226 |
| Rwork | 0.219 |
| R-free | 0.29660 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.003 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.702 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 25.000 | 4.220 |
| 分解能 [Å] (高) | 3.850 | 3.850 |
| Rpim | 0.076 | 0.727 |
| 独立反射数 | 19830 | 4618 |
| <I/σ(I)> | 7.6 | |
| 完全性 [%] | 99.6 | |
| 冗長性 | 9.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 32% PEG400, 0.05 M sodium acetate, 0.1 M magnesium acetate, 3.6 mM decyl beta-D-maltoside |
