6OE5
Splayed open prefusion RSV F captured by CR9501 and motavizumab Fabs
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-26 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 61 |
格子定数 [Å] | 219.837, 219.837, 68.811 |
格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
残基 | 43.677 - 4.100 |
R因子 | 0.2637 |
Rwork | 0.262 |
R-free | 0.30570 |
Structure solution method | MOLECULAR REPLACEMENT |
結合長の平均二乗偏差(RMSD) [Å] | 0.003 |
結合角の平均二乗偏差(RMSD) [度] | 0.501 |
Data reduction software | iMOSFLM (7.2.1) |
Data scaling software | Aimless (0.5.26) |
Phasing software | PHASER (2.5.7) |
Refinement software | PHENIX (1.12_2829) |
Quality characteristics
Overall | Inner shell | Outer shell | |
分解能 [Å] (低) | 43.680 | 43.680 | 4.580 |
分解能 [Å] (高) | 4.100 | 9.170 | 4.100 |
Rmerge_l_obs | 0.295 | 0.070 | 1.267 |
Rmeas | 0.318 | 0.076 | 1.366 |
Rpim | 0.116 | 0.028 | 0.505 |
Total number of observations | 110763 | ||
独立反射数 | 15219 | 1412 | 4299 |
<I/σ(I)> | 5.3 | ||
完全性 [%] | 99.9 | 98.8 | 100 |
冗長性 | 7.3 | 7 | 7.1 |
CC(1/2) | 0.988 | 0.988 | 0.607 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 4.69 mg/mL prefusion F (PR-DM) + motavizumab Fab + CR9501 Fab, 30% (v/v) PEG400, 0.19 M ammonium sulfate, 3.1% (w/v) PEG8000, 0.1 M Tris pH 8.5 |