6O8B
Crystal structure of STING CTD in complex with TBK1
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2017-04-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| 格子定数 [Å] | 250.690, 250.690, 239.241 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 82.058 - 3.400 |
| R因子 | 0.2442 |
| Rwork | 0.243 |
| R-free | 0.25780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4im2 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.002 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.577 |
| Data reduction software | iMOSFLM |
| Data scaling software | pointless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 82.058 | 3.490 |
| 分解能 [Å] (高) | 3.400 | 3.400 |
| Rmerge_l_obs | 0.152 | 2.000 |
| Rpim | 0.063 | 0.860 |
| 独立反射数 | 60675 | 4434 |
| <I/σ(I)> | 7.8 | |
| 完全性 [%] | 99.3 | 5.9 |
| 冗長性 | 6.2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 0.8 M (NH4)2SO4, 0.1 M BIS-TRIS pH 5.5 and 1% PEG3350 |
