6O3N
Cross-alpha Amyloid-like Structure alphaAmA
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.11583 |
| Spacegroup name | I 41 2 2 |
| 格子定数 [Å] | 164.816, 164.816, 164.134 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 116.500 - 3.700 |
| R因子 | 0.29888 |
| Rwork | 0.297 |
| R-free | 0.31925 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6c4z |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.681 |
| Data reduction software | XDS (VERSION Jan 26, 2018 BUILT=20180409) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 116.500 | 3.930 |
| 分解能 [Å] (高) | 3.700 | 3.700 |
| Rmeas | 0.098 | 2.110 |
| 独立反射数 | 12514 | 1946 |
| <I/σ(I)> | 18.48 | 1.59 |
| 完全性 [%] | 99.7 | 98 |
| 冗長性 | 25.6 | 25.5 |
| CC(1/2) | 0.997 | 0.465 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 45% MPD, 0.25M NaH2PO4 |
