6NSB
Crystal structure of the IVR-165 (H3N2) influenza virus hemagglutinin in complex with 6'-SLNLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 101.123, 101.123, 387.229 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.750 |
| R-factor | 0.16594 |
| Rwork | 0.165 |
| R-free | 0.18686 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o5n |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.529 |
| Data scaling software | HKL-2000 (712) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rpim | 0.020 | 0.230 |
| Number of reflections | 76888 | 6920 |
| <I/σ(I)> | 39 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 37.7 | |
| CC(1/2) | 1.000 | 0.920 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 44% 2-methyl-2,4-pentanediol, 0.1 M HEPES pH 7.0 |
