6NSA
Crystal structure of the IVR-165 (H3N2) influenza virus hemagglutinin in complex with 3'-SLNLN
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-08-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9765 |
| Spacegroup name | H 3 2 |
| 格子定数 [Å] | 101.462, 101.462, 388.077 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 50.000 - 1.950 |
| R因子 | 0.18084 |
| Rwork | 0.179 |
| R-free | 0.20757 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o5n |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.515 |
| Data scaling software | HKL-2000 (712) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0158) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.020 |
| 分解能 [Å] (高) | 1.950 | 1.950 |
| Rpim | 0.040 | 0.380 |
| 独立反射数 | 56657 | 5556 |
| <I/σ(I)> | 33.5 | |
| 完全性 [%] | 99.7 | 99.4 |
| 冗長性 | 7.5 | 7.5 |
| CC(1/2) | 1.000 | 0.860 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 44% 2-methyl-2,4-pentanediol, 0.1 M HEPES pH 7.0 |
