6NOS

PD-L1 IgV domain V76T with fragment

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-28
Detector	RAYONIX MX-300
Wavelength(s)	1
Spacegroup name	P 21 21 2
格子定数 [Å]	84.014, 97.401, 33.182
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	29.421 - 2.701
R因子	0.1944
Rwork	0.185
R-free	0.27540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i8l
結合長の平均二乗偏差(RMSD) [Å]	0.010
結合角の平均二乗偏差(RMSD) [度]	1.415
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	40.000	2.750
分解能 [Å] (高)	2.700	2.700
Rmerge_l_obs	0.129	0.469
Rmeas	0.146	0.566
Rpim	0.066	0.309
独立反射数	7548	354
<I/σ(I)>	7.2
完全性 [%]	94.2	89.6
冗長性	4.1	2.6
CC(1/2)	0.079

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	EVAPORATION		291	0.056 M sodium phosphate monobasic, 1.344 M potassium phosphate dibasic