6NHG
Rhodobacter sphaeroides Mitochondrial respiratory chain complex
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.739 |
| Spacegroup name | I 41 2 2 |
| 格子定数 [Å] | 154.181, 154.181, 598.180 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 29.870 - 2.800 |
| R因子 | 0.2518 |
| Rwork | 0.251 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sqx |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 30.000 | 2.850 |
| 分解能 [Å] (高) | 2.800 | 7.560 | 2.800 |
| Rmerge_l_obs | 0.104 | 0.044 | 1.189 |
| Rmeas | 0.107 | 0.045 | 1.230 |
| Rpim | 0.022 | 0.009 | 0.310 |
| 独立反射数 | 167324 | 4740 | 4392 |
| <I/σ(I)> | 10 | ||
| 完全性 [%] | 99.8 | 98.1 | 100 |
| 冗長性 | 23.2 | 21.8 | 15.2 |
| CC(1/2) | 0.999 | 0.889 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 277 | 50 mM MOPS 7.2 pH, 20 mM Ammonium acetate, 20% glycerol. Incubation with 2-5 mol excess of Azoxystrobin. Precipitant 12% PEG4000, 0.5 M KCl, 0.1% DHPC; Protein:PPT ratio 1:0.57 |
