6NC8

Lipid II flippase MurJ, inward occluded conformation

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.65
Spacegroup name	P 2 21 21
格子定数 [Å]	68.419, 80.261, 100.687
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	68.419 - 2.601
R因子	0.2302
Rwork	0.229
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t77
結合長の平均二乗偏差(RMSD) [Å]	0.003
結合角の平均二乗偏差(RMSD) [度]	0.625
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	100.000	2.700
分解能 [Å] (高)	2.600	2.600
独立反射数	17462
<I/σ(I)>	13.29
完全性 [%]	99.8
冗長性	48.8

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	LIPIDIC CUBIC PHASE	5	295	100 mM Na citrate tribasic-HCl pH 5.0, 40% PEG200