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6N9F

Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic ACCA-DPhe and bound to mRNA and P-site tRNA at 3.7A resolution

This is a non-PDB format compatible entry.
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 24-ID-C
Synchrotron siteAPS
Beamline24-ID-C
Temperature [K]100
Detector technologyPIXEL
Collection date2018-03-24
DetectorDECTRIS PILATUS 6M-F
Wavelength(s)0.97950
Spacegroup nameP 21 21 21
Unit cell lengths211.390, 452.150, 617.640
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution255.015 - 3.700
R-factor0.2382
Rwork0.236
R-free0.27940
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4y4p
RMSD bond length0.003
RMSD bond angle0.635
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwarePHENIX (1.8.2)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]255.015255.0153.800
High resolution limit [Å]3.70016.5503.700
Rmerge0.1770.0431.437
Rmeas0.2090.0511.727
Total number of observations2154810
Number of reflections615531706945204
<I/σ(I)>5.5926.60.86
Completeness [%]98.59498.5
Redundancy3.5013.6773.143
CC(1/2)0.9950.9950.249
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION7.62920.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME

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건을2024-03-27부터공개중

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