6N9E
Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution
これはPDB形式変換不可エントリーです。
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 212.240, 452.840, 620.300 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 146.711 - 3.700 |
| R因子 | 0.2388 |
| Rwork | 0.237 |
| R-free | 0.27770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y4p |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.003 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.635 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 146.711 | 146.711 | 3.800 |
| 分解能 [Å] (高) | 3.700 | 16.550 | 3.700 |
| Rmerge_l_obs | 0.237 | 0.048 | 2.508 |
| Rmeas | 0.253 | 0.052 | 2.690 |
| Total number of observations | 5428817 | ||
| 独立反射数 | 627226 | 7395 | 45894 |
| <I/σ(I)> | 6.87 | 39.25 | 0.88 |
| 完全性 [%] | 99.4 | 97.2 | 99 |
| 冗長性 | 8.655 | 8.919 | 7.946 |
| CC(1/2) | 0.997 | 0.997 | 0.188 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME |
