6N9E

Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	212.240, 452.840, 620.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	146.711 - 3.700
R-factor	0.2388
Rwork	0.237
R-free	0.27770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4p
RMSD bond length	0.003
RMSD bond angle	0.635
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	146.711	146.711	3.800
High resolution limit [Å]	3.700	16.550	3.700
Rmerge	0.237	0.048	2.508
Rmeas	0.253	0.052	2.690
Total number of observations	5428817
Number of reflections	627226	7395	45894
<I/σ(I)>	6.87	39.25	0.88
Completeness [%]	99.4	97.2	99
Redundancy	8.655	8.919	7.946
CC(1/2)	0.997	0.997	0.188

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME