6N5W

Crystal structure of the Ca2+/CaM complex with independent peptides of Kv7.4 (KCNQ4) A & B domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	43.510, 130.570, 36.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.278 - 2.150
R-factor	0.2367
Rwork	0.233
R-free	0.27390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4umo
RMSD bond length	0.010
RMSD bond angle	1.127
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.520	2.270
High resolution limit [Å]	2.150	2.150
Rpim	0.060	0.404
Number of reflections	11851	1702
<I/σ(I)>	8.1	2
Completeness [%]	100.0	100
Redundancy	7.9	7.9
CC(1/2)	0.995	0.535

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	1.3 M sodium citrate, 0.1 M HEPES pH 7.0