6M97

Crystal structure of the high-affinity copper transporter Ctr1

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.2546
Spacegroup name	H 3 2
格子定数 [Å]	73.850, 73.850, 410.580
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	20.000 - 3.030
R因子	0.2854
Rwork	0.280
R-free	0.33231
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	1.315
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0158)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	3.110
分解能 [Å] (高)	3.000	3.000
Rmerge_l_obs	0.121	1.578
Rpim	0.057	0.732
独立反射数	8991	890
<I/σ(I)>	16.8
完全性 [%]	99.3
冗長性	5.5
CC(1/2)		0.747

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP		293	50 mM zinc acetate, 50 mM sodium cacodylate pH 5.9, and 26% PEG 400.