6K1E
Crystal structure of EXD2 exonuclease domain soaked in Mg and GMP
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97936 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 46.496, 73.288, 134.086 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 49.467 - 2.900 |
| R因子 | 0.23 |
| Rwork | 0.228 |
| R-free | 0.26840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6k17 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.051 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.950 |
| 分解能 [Å] (高) | 2.900 | 2.900 |
| Rmerge_l_obs | 0.109 | 0.343 |
| 独立反射数 | 10440 | 467 |
| <I/σ(I)> | 13.54 | |
| 完全性 [%] | 96.6 | |
| 冗長性 | 3.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 8000, 2-(N-morpholino)ethanesulfonic acid, Calcium acetate, Dithiothreitol, Magnesium chloride, guanosine monophosphate |
