6JYN
GII.13/21 noroviruses recognize glycans with a terminal beta-galactose via an unconventional glycan binding site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 113 |
| Detector technology | PIXEL |
| Collection date | 2018-09-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97774 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.936, 122.935, 83.280 |
| Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
| Resolution | 49.455 - 1.599 |
| R-factor | 0.1864 |
| Rwork | 0.181 |
| R-free | 0.20070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.599 | 1.600 |
| Number of reflections | 184076 | 184076 |
| <I/σ(I)> | 18.333 | |
| Completeness [%] | 99.5 | 97.5 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M sodium citrate tribasic dehydrate, 20 percent polyethylene glycol 3350 |
