6JP2
Crystal structure of pyrrolysyl-tRNA synthetase from Methanomethylophilus alvus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-23 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.380, 112.780, 107.430 |
| Unit cell angles | 90.00, 114.21, 90.00 |
Refinement procedure
| Resolution | 48.991 - 2.272 |
| R-factor | 0.2152 |
| Rwork | 0.213 |
| R-free | 0.25670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zce |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.513 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 2.310 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmeas | 0.089 | 0.676 |
| Number of reflections | 55681 | 2345 |
| <I/σ(I)> | 19.1 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG3350, Cesium Chloride |
