6JP2
Crystal structure of pyrrolysyl-tRNA synthetase from Methanomethylophilus alvus
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-23 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 112.380, 112.780, 107.430 |
| 格子定数 [度] | 90.00, 114.21, 90.00 |
精密化法
| 残基 | 48.991 - 2.272 |
| R因子 | 0.2152 |
| Rwork | 0.213 |
| R-free | 0.25670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zce |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.002 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.513 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 49.000 | 2.310 |
| 分解能 [Å] (高) | 2.270 | 2.270 |
| Rmeas | 0.089 | 0.676 |
| 独立反射数 | 55681 | 2345 |
| <I/σ(I)> | 19.1 | |
| 完全性 [%] | 99.0 | |
| 冗長性 | 3.7 | 3.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG3350, Cesium Chloride |
