6JKW
Seleno-methionine PNGM-1 from deep-sea sediment metagenome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 121.857, 83.124, 162.833 |
| Unit cell angles | 90.00, 110.18, 90.00 |
Refinement procedure
| Resolution | 34.930 - 2.290 |
| R-factor | 0.2143 |
| Rwork | 0.211 |
| R-free | 0.27420 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.725 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.930 | 2.340 |
| High resolution limit [Å] | 2.290 | 2.300 |
| Number of reflections | 137843 | |
| <I/σ(I)> | 4.7 | |
| Completeness [%] | 98.1 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 287 | 0.1M Tris-HCl, 0.2M MgCl2 and 10% PEG 8000 |
