6JGH
Crystal structure of the F99S/M153T/V163A/T203I variant of GFP at 0.94 A
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 56 |
Detector technology | CCD |
Collection date | 2016-12-13 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.75 |
Spacegroup name | P 21 21 21 |
格子定数 [Å] | 50.641, 62.506, 68.179 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 29.928 - 0.940 |
R因子 | 0.108 |
Rwork | 0.107 |
R-free | 0.12900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wur |
Data reduction software | DENZO |
Data scaling software | SDMS |
Phasing software | PHASER |
Refinement software | SHELXL |
Quality characteristics
Overall | Inner shell | Outer shell | |
分解能 [Å] (低) | 50.000 | 50.000 | 0.960 |
分解能 [Å] (高) | 0.940 | 2.550 | 0.940 |
Rmerge_l_obs | 0.147 | 0.064 | 1.260 |
Rmeas | 0.154 | 0.067 | 1.358 |
Rpim | 0.047 | 0.019 | 0.503 |
独立反射数 | 141942 | 7529 | 6969 |
<I/σ(I)> | 5.1 | ||
完全性 [%] | 99.8 | 99.9 | 99.3 |
冗長性 | 8.9 | 11.9 | 7.1 |
CC(1/2) | 0.998 | 0.542 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION | 8.5 | 308 | PEG 4000, MgCl2, Tris-HCl buffer |