6J7L
Crystal structure of Pseudomonas aeruginosa Earp in complex with TDP
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.988 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 45.322, 92.002, 119.138 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 38.478 - 1.851 |
| R因子 | 0.1708 |
| Rwork | 0.169 |
| R-free | 0.20150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6j7j |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.812 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 40.000 | 40.000 | 1.920 |
| 分解能 [Å] (高) | 1.850 | 3.980 | 1.850 |
| Rmerge_l_obs | 0.075 | 0.042 | 0.690 |
| Rmeas | 0.078 | 0.044 | 0.717 |
| Rpim | 0.021 | 0.012 | 0.192 |
| Total number of observations | 567700 | ||
| 独立反射数 | 43403 | 4620 | 4266 |
| <I/σ(I)> | 4.1 | ||
| 完全性 [%] | 100.0 | 100 | 100 |
| 冗長性 | 13.1 | 12.4 | 13.5 |
| CC(1/2) | 0.999 | 0.931 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES pH6.5, 12% PEG20K |
