6J7J
Crystal structure of Pseudomonas aeruginosa Earp
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 45.217, 93.183, 119.886 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 38.523 - 1.750 |
| R因子 | 0.1769 |
| Rwork | 0.175 |
| R-free | 0.20910 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.14_3260) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 40.000 | 40.000 | 1.780 |
| 分解能 [Å] (高) | 1.750 | 4.750 | 1.750 |
| Rmerge_l_obs | 0.083 | 0.050 | 0.993 |
| Rmeas | 0.090 | 0.055 | 1.070 |
| Rpim | 0.033 | 0.021 | 0.397 |
| Total number of observations | 369193 | ||
| 独立反射数 | 51672 | 2757 | 2548 |
| <I/σ(I)> | 8.7 | ||
| 完全性 [%] | 98.9 | 96.7 | 99.8 |
| 冗長性 | 7.1 | 6.5 | 7 |
| CC(1/2) | 0.997 | 0.791 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES pH6.5, 12% PEG20K |
