6IV8
the selenomethionine(SeMet)-derived Cas13d binary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-05-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.164, 145.733, 249.383 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.920 - 2.150 |
R-factor | 0.1898 |
Rwork | 0.189 |
R-free | 0.21030 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.781 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.920 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.129 | |
Rpim | 0.053 | 0.337 |
Number of reflections | 129626 | 6409 |
<I/σ(I)> | 14.5 | |
Completeness [%] | 99.7 | 100 |
Redundancy | 13.3 | |
CC(1/2) | 0.998 | 0.939 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | (0.5% (w/v) Tryptone, 0.1 M HEPES sodium (pH 7.0), 16% (w/v) Polyethylene glycol 3350) |