6IV8

the selenomethionine(SeMet)-derived Cas13d binary complex

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
格子定数 [Å]	65.164, 145.733, 249.383
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	62.920 - 2.150
R因子	0.1898
Rwork	0.189
R-free	0.21030
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.013
結合角の平均二乗偏差(RMSD) [度]	1.781
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0222)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	62.920	2.190
分解能 [Å] (高)	2.150	2.150
Rmerge_l_obs	0.129
Rpim	0.053	0.337
独立反射数	129626	6409
<I/σ(I)>	14.5
完全性 [%]	99.7	100
冗長性	13.3
CC(1/2)	0.998	0.939

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP		289	(0.5% (w/v) Tryptone, 0.1 M HEPES sodium (pH 7.0), 16% (w/v) Polyethylene glycol 3350)