6IOT
The ligand binding domain of Mlp24 with arginine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-04-19 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.530, 102.750, 93.410 |
Unit cell angles | 90.00, 94.51, 90.00 |
Refinement procedure
Resolution | 48.483 - 2.700 |
R-factor | 0.2193 |
Rwork | 0.217 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.001 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.500 | 2.830 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.103 | 0.359 |
Number of reflections | 32455 | 4264 |
<I/σ(I)> | 8.7 | 3.7 |
Completeness [%] | 97.1 | 96.2 |
Redundancy | 3.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M MES pH 6.0, 45% (v/v) PEG 400, 0.1M calcium acetate |