6IOR
The ligand binding domain of Mlp24 with asparagine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-10-05 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 91.880, 101.350, 128.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 68.071 - 2.500 |
R-factor | 0.2018 |
Rwork | 0.199 |
R-free | 0.25630 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.212 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.600 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.121 | 0.558 |
Number of reflections | 42225 | 4375 |
<I/σ(I)> | 8.5 | 2.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M MES pH6.0, 50% (v/v) PEG 400, 0.1M calcium acetate |