6IEJ

The C2 domain of cytosolic phospholipase A2 alpha bound to phosphatidylcholine

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	C 2 2 2
格子定数 [Å]	108.294, 187.388, 68.754
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	46.881 - 2.206
R因子	0.2256
Rwork	0.224
R-free	0.24890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rlw
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	1.117
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.240
分解能 [Å] (高)	2.200	2.200
独立反射数	35668
<I/σ(I)>	30.9
完全性 [%]	99.5
冗長性	7.7

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION	7	293	100 mM HEPES-NaOH buffer, 1.4 M magnesium chloride, 0.6 M sodium chloride